| 27. Journée | 29.01.2021 | Colchester United | - | Scunthorpe United | 0:1 | ||||
| 28. Journée | 06.02.2021 | Leyton Orient | - | Colchester United | 0:0 | ||||
| 29. Journée | 14.02.2021 | Colchester United | - | Mansfield Town | 2:2 | ||||
| 32. Journée | 27.02.2021 | Forest Green Rovers | - | Colchester United | 3:0 | ||||
| 33. Journée | 02.03.2021 | Colchester United | - | Carlisle United | 2:1 | ||||
| 34. Journée | 06.03.2021 | Colchester United | - | Newport County | 0:2 | ||||
| 35. Journée | 09.03.2021 | Harrogate Town | - | Colchester United | 3:0 | ||||
| 36. Journée | 13.03.2021 | Grimsby Town | - | Colchester United | 0:0 | ||||
| 23. Journée | 16.03.2021 | Salford City | - | Colchester United | 0:0 | ||||
| 37. Journée | 20.03.2021 | Colchester United | - | Port Vale FC | 0:1 | ||||
| 42. Journée | 17.04.2021 | Colchester United | - | Walsall FC | 2:1 |  |
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| 43. Journée | 20.04.2021 | Colchester United | - | Southend United | 2:0 | ||||
| 44. Journée | 24.04.2021 | Cheltenham Town | - | Colchester United | 1:0 | ||||
| 45. Journée | 01.05.2021 | Colchester United | - | Salford City | 1:0 | ||||
| 46. Journée | 08.05.2021 | Tranmere Rovers | - | Colchester United | 0:0 |
Angleterre