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| 09.04.2010 | 20:00 | Fortuna Sittard | - | Go Ahead Eagles | 2:0 (1:0) | |
| 20:00 | FC Zwolle | - | FC Volendam | 4:2 (2:2) | ||
| 20:00 | FC Omniworld | - | De Graafschap | 0:5 (0:0) | ||
| 20:00 | SC Cambuur | - | FC Den Bosch | 1:0 (1:0) | ||
| 20:00 | FC Dordrecht | - | Helmond Sport | 0:1 (0:1) | ||
| 20:00 | MVV | - | RBC Roosendaal | 2:1 (2:1) | ||
| 20:00 | Telstar | - | BV Veendam | 1:1 (1:1) | ||
| 20:00 | FC Oss | - | AGOVV | 1:0 (0:0) | ||
| 20:00 | SBV Excelsior | - | FC Eindhoven | 4:1 (1:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 34 | 24 | 6 | 4 | 76:27 | 49 | 78 |
| 2 |
|
SC Cambuur | 34 | 20 | 8 | 6 | 76:47 | 29 | 68 |
| 3 |
|
SBV Excelsior | 34 | 18 | 5 | 11 | 67:46 | 21 | 59 |
| 4 |
|
Go Ahead Eagles | 34 | 17 | 8 | 9 | 49:31 | 18 | 59 |
| 5 |
|
FC Zwolle | 34 | 17 | 8 | 9 | 53:37 | 16 | 59 |
| 6 |
|
FC Den Bosch | 35 | 14 | 12 | 9 | 65:49 | 16 | 54 |
| 7 |
|
AGOVV | 34 | 15 | 9 | 10 | 58:47 | 11 | 52 |
| 8 |
|
Helmond Sport | 34 | 15 | 6 | 13 | 61:51 | 10 | 51 |
| 9 |
|
BV Veendam | 34 | 13 | 10 | 11 | 55:47 | 8 | 48 |
| 10 |
|
MVV | 35 | 13 | 7 | 15 | 54:66 | -12 | 45 |
| 11 |
|
RBC Roosendaal | 34 | 11 | 10 | 13 | 45:50 | -5 | 43 |
| 12 |
|
FC Eindhoven | 34 | 12 | 7 | 15 | 59:74 | -15 | 43 |
| 13 |
|
FC Omniworld | 34 | 11 | 7 | 16 | 38:57 | -19 | 40 |
| 14 |
|
FC Emmen | 34 | 10 | 8 | 16 | 51:74 | -23 | 38 |
| 15 |
|
FC Dordrecht | 34 | 11 | 4 | 19 | 49:57 | -8 | 37 |
| 16 |
|
FC Volendam | 34 | 7 | 11 | 16 | 56:78 | -22 | 32 |
| 17 |
|
Fortuna Sittard | 34 | 7 | 9 | 18 | 31:53 | -22 | 30 |
| 18 |
|
Telstar | 34 | 7 | 7 | 20 | 43:62 | -19 | 28 |
| 19 |
|
FC Oss | 34 | 6 | 10 | 18 | 40:73 | -33 | 28 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
