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| 26.02.2010 | 20:00 | De Graafschap | - | SBV Excelsior | 3:1 (1:0) | |
| 20:00 | Helmond Sport | - | FC Volendam | 5:2 (2:1) | ||
| 20:00 | FC Zwolle | - | FC Den Bosch | 1:1 (1:0) | ||
| 20:00 | Telstar | - | AGOVV | 1:2 (1:2) | ||
| 20:00 | MVV | - | Go Ahead Eagles | 0:0 (0:0) | ||
| 20:00 | RBC Roosendaal | - | BV Veendam | 2:2 (0:1) | ||
| 20:00 | FC Omniworld | - | FC Eindhoven | 3:1 (1:1) | ||
| 20:00 | FC Oss | - | Fortuna Sittard | 1:1 (0:0) | ||
| 20:00 | FC Emmen | - | SC Cambuur | 2:4 (1:2) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 28 | 18 | 6 | 4 | 56:23 | 33 | 60 |
| 2 |
|
Go Ahead Eagles | 27 | 15 | 8 | 4 | 44:20 | 24 | 53 |
| 3 |
|
SC Cambuur | 27 | 15 | 8 | 4 | 63:41 | 22 | 53 |
| 4 |
|
FC Zwolle | 28 | 15 | 6 | 7 | 44:29 | 15 | 51 |
| 5 |
|
SBV Excelsior | 27 | 14 | 4 | 9 | 51:36 | 15 | 46 |
| 6 |
|
AGOVV | 28 | 13 | 8 | 7 | 49:40 | 9 | 45 |
| 7 |
|
Helmond Sport | 27 | 13 | 5 | 9 | 55:43 | 12 | 44 |
| 8 |
|
FC Den Bosch | 28 | 10 | 10 | 8 | 51:41 | 10 | 40 |
| 9 |
|
BV Veendam | 28 | 11 | 8 | 9 | 46:38 | 8 | 40 |
| 10 |
|
RBC Roosendaal | 28 | 10 | 7 | 11 | 34:40 | -6 | 37 |
| 11 |
|
MVV | 28 | 10 | 6 | 12 | 45:52 | -7 | 35 |
| 12 |
|
FC Eindhoven | 27 | 10 | 5 | 12 | 49:59 | -10 | 35 |
| 13 |
|
FC Dordrecht | 27 | 10 | 3 | 14 | 46:46 | 0 | 33 |
| 14 |
|
FC Omniworld | 27 | 8 | 5 | 14 | 30:48 | -18 | 29 |
| 15 |
|
FC Volendam | 27 | 6 | 10 | 11 | 46:56 | -10 | 28 |
| 16 |
|
FC Emmen | 28 | 7 | 5 | 16 | 37:64 | -27 | 26 |
| 17 |
|
Telstar | 27 | 6 | 5 | 16 | 35:50 | -15 | 23 |
| 18 |
|
Fortuna Sittard | 28 | 5 | 7 | 16 | 24:47 | -23 | 22 |
| 19 |
|
FC Oss | 27 | 3 | 8 | 16 | 31:63 | -32 | 17 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotion | |
| Play-off | |
| Play-off |
