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| 17.04.2009 | 20:00 | TOP Oss | - | AGOVV | 1:1 (1:1) | |
| 20:00 | FC Dordrecht | - | FC Omniworld | 2:1 (1:1) | ||
| 20:00 | FC Zwolle | - | Fortuna Sittard | 2:1 (1:1) | ||
| 20:00 | BV Veendam | - | Telstar | 1:0 (1:0) | ||
| 20:00 | Helmond Sport | - | FC Den Bosch | 3:0 (2:0) | ||
| 20:00 | MVV | - | SC Cambuur | 1:2 (1:1) | ||
| 20:00 | HFC Haarlem | - | Go Ahead Eagles | 1:2 (0:1) | ||
| 20:00 | RBC Roosendaal | - | FC Eindhoven | 1:2 (1:0) | ||
| 20:00 | VVV-Venlo | - | FC Emmen | 4:0 (3:0) | ||
| 20:00 | SBV Excelsior | - | RKC Waalwijk | 0:5 (0:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
VVV-Venlo | 35 | 23 | 5 | 7 | 80:35 | 45 | 74 |
| 2 |
|
RKC Waalwijk | 35 | 17 | 17 | 1 | 63:26 | 37 | 68 |
| 3 |
|
SC Cambuur | 35 | 18 | 9 | 8 | 63:47 | 16 | 63 |
| 4 |
|
FC Zwolle | 35 | 17 | 8 | 10 | 55:45 | 10 | 59 |
| 5 |
|
MVV | 35 | 14 | 12 | 9 | 53:37 | 16 | 54 |
| 6 |
|
SBV Excelsior | 35 | 14 | 12 | 9 | 49:41 | 8 | 54 |
| 7 |
|
Go Ahead Eagles | 35 | 12 | 14 | 9 | 37:37 | 0 | 50 |
| 8 |
|
HFC Haarlem | 35 | 15 | 5 | 15 | 41:51 | -10 | 50 |
| 9 |
|
Helmond Sport | 35 | 13 | 10 | 12 | 48:46 | 2 | 49 |
| 10 |
|
FC Den Bosch | 35 | 13 | 8 | 14 | 56:49 | 7 | 47 |
| 11 |
|
FC Dordrecht | 35 | 12 | 10 | 13 | 45:37 | 8 | 46 |
| 12 |
|
AGOVV | 35 | 13 | 7 | 15 | 55:63 | -8 | 46 |
| 13 |
|
TOP Oss | 35 | 11 | 9 | 15 | 49:58 | -9 | 42 |
| 14 |
|
FC Emmen | 35 | 11 | 8 | 16 | 45:66 | -21 | 41 |
| 15 |
|
RBC Roosendaal | 35 | 11 | 7 | 17 | 42:49 | -7 | 40 |
| 16 |
|
Telstar | 35 | 9 | 11 | 15 | 40:52 | -12 | 38 |
| 17 |
|
BV Veendam | 35 | 10 | 9 | 16 | 43:61 | -18 | 36 |
| 18 |
|
Fortuna Sittard | 35 | 9 | 8 | 18 | 36:55 | -19 | 35 |
| 19 |
|
FC Eindhoven | 35 | 7 | 14 | 14 | 54:76 | -22 | 32 |
| 20 |
|
FC Omniworld | 35 | 7 | 5 | 23 | 37:60 | -23 | 26 |
| Promotion | |
| Play-off | |
| Play-off | |
| Play-off | |
| Play-off |
