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| 02.01.2001 | 17:00 | Granada CF | - | Villarreal CF | 4:2 (0:0, 1:1, 1:1) tab | |
| 20:45 | UD SS Reyes | - | Athletic Club | 1:2 (1:0) | ||
| 21:30 | Levante UD | - | Espanyol Barcelona | 0:2 (0:1) | ||
| 03.01.2001 | 20:30 | SD Beasain | - | Real Zaragoza | 0:3 (0:1) | |
| 20:45 | Gimnástica Torrelavega | - | UD Las Palmas | 2:1 (1:0) | ||
| 20:45 | Atlético Madrid | - | CA Osasuna | 3:1 (1:0) | ||
| 21:00 | CD Toledo | - | Rayo Vallecano | 0:1 (0:1) | ||
| 21:00 | CF Extremadura | - | Real Valladolid | 2:1 (1:0, 1:1) ap | ||
| 21:00 | CD Leganés | - | CD Numancia | 2:0 (0:0) | ||
| 21:00 | Xerez CD | - | RCD Mallorca | 0:3 (0:0) | ||
| 21:00 | CD Badajoz | - | Racing Santander | 0:4 (0:1) | ||
| 21:15 | Guadix Club de Fútbol | - | Valencia CF | 6:5 (3:1, 4:4, 4:4) tab | ||
| 21:15 | Real Jaén | - | Real Murcia | 8:9 (1:0, 1:1, 1:1) tab | ||
| 21:30 | SD Compostela | - | RC Celta | 1:3 (0:1, 1:1) ap | ||
| 21:30 | AD Ceuta | - | FC Barcelona | 0:3 (0:0) | ||
| 22:00 | CD Tenerife | - | Deportivo La Coruña | 3:2 (0:1) |
