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| 13.03.2010 | 18:20 | Slavia Praha | - | FK Teplice | 0:0 (0:0) | |
| 14.03.2010 | 15:00 | Dynamo České Budějovice | - | Slovan Liberec | 1:1 (0:0) | |
| 15:00 | FK Baumit Jablonec | - | SK Kladno | 1:0 (1:0) | ||
| 15:00 | Viktoria Plzeň | - | Bohemians 1905 | 3:0 (2:0) | ||
| 17:00 | Bohemians Praha | - | 1. FC Slovácko | 1:1 (1:1) | ||
| 17:00 | FK Mladá Boleslav | - | AC Sparta Praha | 1:2 (1:0) | ||
| 17:00 | Sigma Olomouc | - | 1. FK Příbram | 3:1 (1:1) | ||
| 15.03.2010 | 17:30 | 1. FC Brno | - | Baník Ostrava | 1:1 (0:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Teplice | 19 | 11 | 7 | 1 | 33:18 | 15 | 40 |
| 2 |
|
AC Sparta Praha | 19 | 10 | 9 | 0 | 23:9 | 14 | 39 |
| 3 |
|
FK Baumit Jablonec | 19 | 11 | 4 | 4 | 22:16 | 6 | 37 |
| 4 |
|
Baník Ostrava | 19 | 10 | 6 | 3 | 28:17 | 11 | 36 |
| 5 |
|
Viktoria Plzeň | 19 | 9 | 6 | 4 | 23:15 | 8 | 33 |
| 6 |
|
Slavia Praha | 19 | 8 | 7 | 4 | 25:16 | 9 | 31 |
| 7 |
|
Sigma Olomouc | 19 | 8 | 4 | 7 | 31:20 | 11 | 28 |
| 8 |
|
FK Mladá Boleslav | 19 | 7 | 5 | 7 | 31:26 | 5 | 26 |
| 9 |
|
1. FK Příbram | 19 | 7 | 4 | 8 | 21:23 | -2 | 25 |
| 10 |
|
1. FC Brno | 19 | 6 | 6 | 7 | 17:23 | -6 | 24 |
| 11 |
|
Slovan Liberec | 19 | 6 | 3 | 10 | 19:26 | -7 | 21 |
| 12 |
|
Bohemians 1905 | 19 | 5 | 5 | 9 | 11:19 | -8 | 20 |
| 13 |
|
Dynamo České Budějovice | 19 | 3 | 7 | 9 | 16:25 | -9 | 16 |
| 14 |
|
1. FC Slovácko | 19 | 4 | 3 | 12 | 15:27 | -12 | 15 |
| 15 |
|
SK Kladno | 19 | 4 | 2 | 13 | 13:29 | -16 | 14 |
| 16 |
|
Bohemians Praha | 19 | 3 | 2 | 14 | 17:36 | -19 | -9 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
