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| 17.10.2009 | 16:00 | 1. FK Příbram | - | FK Teplice | 0:3 (0:1) | |
| 18:00 | AC Sparta Praha | - | Dynamo České Budějovice | 1:1 (1:0) | ||
| 18:15 | Baník Ostrava | - | Slovan Liberec | 1:0 (0:0) | ||
| 18.10.2009 | 16:00 | FK Mladá Boleslav | - | Bohemians Praha | 4:2 (1:1) | |
| 17:00 | Bohemians 1905 | - | 1. FC Slovácko | 0:1 (0:0) | ||
| 17:00 | SK Kladno | - | Slavia Praha | 0:2 (0:1) | ||
| 17:00 | Sigma Olomouc | - | 1. FC Brno | 5:0 (3:0) | ||
| 19.10.2009 | 17:30 | FK Baumit Jablonec | - | Viktoria Plzeň | 0:1 (0:1) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Teplice | 11 | 7 | 4 | 0 | 22:8 | 14 | 25 |
| 2 |
|
FK Baumit Jablonec | 11 | 7 | 2 | 2 | 13:9 | 4 | 23 |
| 3 |
|
Baník Ostrava | 11 | 6 | 3 | 2 | 14:10 | 4 | 21 |
| 4 |
|
Viktoria Plzeň | 11 | 6 | 2 | 3 | 13:10 | 3 | 20 |
| 5 |
|
Slavia Praha | 11 | 5 | 4 | 2 | 16:9 | 7 | 19 |
| 6 |
|
AC Sparta Praha | 11 | 4 | 7 | 0 | 10:4 | 6 | 19 |
| 7 |
|
1. FC Brno | 11 | 4 | 4 | 3 | 10:12 | -2 | 16 |
| 8 |
|
FK Mladá Boleslav | 11 | 4 | 3 | 4 | 17:12 | 5 | 15 |
| 9 |
|
1. FK Příbram | 11 | 4 | 2 | 5 | 11:13 | -2 | 14 |
| 10 |
|
Bohemians 1905 | 11 | 2 | 5 | 4 | 5:6 | -1 | 11 |
| 11 |
|
Slovan Liberec | 11 | 3 | 2 | 6 | 13:16 | -3 | 11 |
| 12 |
|
SK Kladno | 11 | 3 | 2 | 6 | 9:16 | -7 | 11 |
| 13 |
|
Sigma Olomouc | 11 | 2 | 4 | 5 | 13:12 | 1 | 10 |
| 14 |
|
1. FC Slovácko | 11 | 3 | 1 | 7 | 9:16 | -7 | 10 |
| 15 |
|
Dynamo České Budějovice | 11 | 1 | 4 | 6 | 6:16 | -10 | 7 |
| 16 |
|
Bohemians Praha | 11 | 2 | 1 | 8 | 9:21 | -12 | -13 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
