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| 25.04.2009 | 17:00 | Viktoria Plzeň | - | Bohemians Praha | 3:1 (0:0) | |
| 17:00 | 1. FK Příbram | - | AC Sparta Praha | 1:1 (0:1) | ||
| 26.04.2009 | 10:15 | Viktoria Žižkov | - | SK Kladno | 2:2 (0:2) | |
| 17:00 | Baník Ostrava | - | Tescoma Zlín | 1:3 (1:1) | ||
| 17:00 | FK Teplice | - | FK Baumit Jablonec | 3:0 (1:0) | ||
| 17:00 | Dynamo České Budějovice | - | 1. FC Brno | 1:1 (1:0) | ||
| 20:00 | Slovan Liberec | - | Sigma Olomouc | 0:1 (0:0) | ||
| 27.04.2009 | 19:00 | Slavia Praha | - | FK Mladá Boleslav | 3:3 (1:2) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 25 | 15 | 7 | 3 | 49:21 | 28 | 52 |
| 2 |
|
AC Sparta Praha | 25 | 12 | 8 | 5 | 39:21 | 18 | 44 |
| 3 |
|
FK Mladá Boleslav | 25 | 11 | 9 | 5 | 34:28 | 6 | 42 |
| 4 |
|
Slovan Liberec | 25 | 11 | 8 | 6 | 31:24 | 7 | 41 |
| 5 |
|
Sigma Olomouc | 25 | 11 | 7 | 7 | 31:31 | 0 | 40 |
| 6 |
|
FK Teplice | 25 | 11 | 6 | 8 | 29:17 | 12 | 39 |
| 7 |
|
FK Baumit Jablonec | 25 | 11 | 4 | 10 | 25:29 | -4 | 37 |
| 8 |
|
Baník Ostrava | 25 | 10 | 5 | 10 | 34:29 | 5 | 35 |
| 9 |
|
Viktoria Plzeň | 25 | 8 | 9 | 8 | 38:32 | 6 | 33 |
| 10 |
|
1. FC Brno | 25 | 8 | 7 | 10 | 26:28 | -2 | 31 |
| 11 |
|
Dynamo České Budějovice | 25 | 5 | 15 | 5 | 23:29 | -6 | 30 |
| 12 |
|
Bohemians Praha | 25 | 8 | 4 | 13 | 27:36 | -9 | 28 |
| 13 |
|
SK Kladno | 25 | 7 | 6 | 12 | 19:37 | -18 | 27 |
| 14 |
|
1. FK Příbram | 25 | 6 | 7 | 12 | 23:33 | -10 | 25 |
| 15 |
|
Viktoria Žižkov | 25 | 4 | 7 | 14 | 23:34 | -11 | 19 |
| 16 |
|
Tescoma Zlín | 25 | 4 | 7 | 14 | 18:40 | -22 | 19 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
