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| 22.02.2009 | 10:15 | Viktoria Žižkov | - | Tescoma Zlín | 1:1 (0:1) | |
| 15:00 | FK Teplice | - | Slovan Liberec | 1:0 (1:0) | ||
| 15:00 | Dynamo České Budějovice | - | 1. FK Příbram | 2:2 (1:1) | ||
| 15:00 | SK Kladno | - | Bohemians Praha | 1:0 (1:0) | ||
| 15:25 | 1. FC Brno | - | Baník Ostrava | 2:0 (1:0) | ||
| 17:00 | Sigma Olomouc | - | AC Sparta Praha | 0:1 (0:0) | ||
| 20:00 | FK Mladá Boleslav | - | FK Baumit Jablonec | 1:2 (0:1) | ||
| 23.02.2009 | 17:15 | Slavia Praha | - | Viktoria Plzeň | 3:2 (2:0) |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 17 | 12 | 4 | 1 | 40:13 | 27 | 40 |
| 2 |
|
FK Mladá Boleslav | 17 | 9 | 4 | 4 | 24:18 | 6 | 31 |
| 3 |
|
Baník Ostrava | 17 | 9 | 3 | 5 | 30:18 | 12 | 30 |
| 4 |
|
AC Sparta Praha | 17 | 9 | 3 | 5 | 28:17 | 11 | 30 |
| 5 |
|
Slovan Liberec | 17 | 7 | 5 | 5 | 21:18 | 3 | 26 |
| 6 |
|
Sigma Olomouc | 17 | 7 | 5 | 5 | 21:22 | -1 | 26 |
| 7 |
|
FK Teplice | 17 | 7 | 4 | 6 | 18:14 | 4 | 25 |
| 8 |
|
FK Baumit Jablonec | 17 | 7 | 4 | 6 | 18:21 | -3 | 25 |
| 9 |
|
1. FC Brno | 17 | 6 | 5 | 6 | 19:16 | 3 | 23 |
| 10 |
|
Viktoria Plzeň | 17 | 5 | 7 | 5 | 25:22 | 3 | 22 |
| 11 |
|
1. FK Příbram | 17 | 5 | 4 | 8 | 19:23 | -4 | 19 |
| 12 |
|
Bohemians Praha | 17 | 6 | 1 | 10 | 23:30 | -7 | 19 |
| 13 |
|
Dynamo České Budějovice | 17 | 3 | 10 | 4 | 16:24 | -8 | 19 |
| 14 |
|
SK Kladno | 17 | 4 | 4 | 9 | 10:27 | -17 | 16 |
| 15 |
|
Viktoria Žižkov | 17 | 3 | 3 | 11 | 17:27 | -10 | 12 |
| 16 |
|
Tescoma Zlín | 17 | 2 | 4 | 11 | 9:28 | -19 | 10 |
| Champions League Quali. | |
| Europa League | |
| Relégation |
