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| 04.04.2025 | 18:00 | Vysočina Jihlava | - | 1. SK Prostějov | 3:0 (2:0) | |
| 05.04.2025 | 14:00 | Zbrojovka Brno | - | MFK Vyškov | 1:2 (0:0) | |
| 14:00 | SFC Opava | - | FK MAS Táborsko | 2:1 (2:0) | ||
| 06.04.2025 | 10:15 | Slavia Praha B | - | FC Zlín | 0:1 (0:0) | |
| 10:30 | AC Sparta Praha B | - | MFK Chrudim | 2:2 (2:1) | ||
| 16:30 | FK Varnsdorf | - | SK Líšeň | 2:1 (2:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | Viktoria Žižkov | 1:0 (1:0) | ||
| 07.04.2025 | 17:00 | Baník Ostrava B | - | Sigma Olomouc B | 3:0 (0:0) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Zlín | 21 | 17 | 4 | 0 | 35:8 | 27 | 55 |
| 2 |
|
MFK Chrudim | 21 | 11 | 5 | 5 | 34:20 | 14 | 38 |
| 3 |
|
MFK Vyškov | 21 | 9 | 8 | 4 | 22:15 | 7 | 35 |
| 4 |
|
Viktoria Žižkov | 21 | 8 | 5 | 8 | 33:29 | 4 | 29 |
| 5 |
|
SK Líšeň | 21 | 7 | 8 | 6 | 23:24 | -1 | 29 |
| 6 |
|
Slavia Praha B | 21 | 7 | 7 | 7 | 32:25 | 7 | 28 |
| 7 |
|
FC Sellier & Bellot Vlašim | 21 | 6 | 10 | 5 | 33:30 | 3 | 28 |
| 8 |
|
1. SK Prostějov | 21 | 7 | 7 | 7 | 25:34 | -9 | 28 |
| 9 |
|
FK MAS Táborsko | 21 | 6 | 7 | 8 | 22:22 | 0 | 25 |
| 10 |
|
Vysočina Jihlava | 21 | 6 | 7 | 8 | 25:30 | -5 | 25 |
| 11 |
|
Baník Ostrava B | 21 | 7 | 4 | 10 | 26:34 | -8 | 25 |
| 12 |
|
SFC Opava | 21 | 6 | 6 | 9 | 23:32 | -9 | 24 |
| 13 |
|
AC Sparta Praha B | 21 | 5 | 8 | 8 | 33:35 | -2 | 23 |
| 14 |
|
FK Varnsdorf | 21 | 5 | 5 | 11 | 24:32 | -8 | 20 |
| 15 |
|
Sigma Olomouc B | 21 | 5 | 5 | 11 | 25:34 | -9 | 20 |
| 16 |
|
Zbrojovka Brno | 21 | 4 | 8 | 9 | 23:34 | -11 | 20 |
| Promotion | |
| Relégation | |
| Relégation |
