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| 16.09.2022 | 18:00 | Vysočina Jihlava | - | FK Varnsdorf | 1:2 (1:1) | |
| 17.09.2022 | 10:15 | MFK Chrudim | - | MFK Karviná | 5:1 (1:1) | |
| 10:15 | MFK Vyškov | - | 1. SK Prostějov | 4:1 (1:0) | ||
| 16:30 | FK Viagem Příbram | - | FK Třinec | 3:1 (2:0) | ||
| 18.09.2022 | 10:15 | Sigma Olomouc B | - | FK Dukla Praha | 2:2 (2:1) | |
| 10:30 | AC Sparta Praha B | - | SFC Opava | 1:0 (1:0) | ||
| 16:30 | FK MAS Táborsko | - | SK Líšeň | 1:0 (0:0) | ||
| 16:30 | FC Sellier & Bellot Vlašim | - | Slavia Praha B | 3:1 (3:1) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
SK Líšeň | 9 | 6 | 2 | 1 | 14:3 | 11 | 20 |
| 2 |
|
MFK Vyškov | 9 | 5 | 2 | 2 | 16:9 | 7 | 17 |
| 3 |
|
FK Viagem Příbram | 9 | 4 | 4 | 1 | 14:9 | 5 | 16 |
| 4 |
|
FK Varnsdorf | 9 | 5 | 1 | 3 | 15:11 | 4 | 16 |
| 5 |
|
Sigma Olomouc B | 9 | 4 | 3 | 2 | 11:11 | 0 | 15 |
| 6 |
|
Vysočina Jihlava | 9 | 4 | 1 | 4 | 13:10 | 3 | 13 |
| 7 |
|
SFC Opava | 9 | 4 | 1 | 4 | 9:7 | 2 | 13 |
| 8 |
|
MFK Karviná | 9 | 4 | 1 | 4 | 15:15 | 0 | 13 |
| 9 |
|
FK Třinec | 9 | 3 | 3 | 3 | 13:13 | 0 | 12 |
| 10 |
|
MFK Chrudim | 9 | 3 | 2 | 4 | 12:11 | 1 | 11 |
| 11 |
|
FK Dukla Praha | 9 | 3 | 2 | 4 | 10:11 | -1 | 11 |
| 12 |
|
FK MAS Táborsko | 9 | 3 | 2 | 4 | 6:14 | -8 | 11 |
| 13 |
|
AC Sparta Praha B | 9 | 3 | 1 | 5 | 10:14 | -4 | 10 |
| 14 |
|
1. SK Prostějov | 9 | 2 | 3 | 4 | 13:19 | -6 | 9 |
| 15 |
|
Slavia Praha B | 9 | 2 | 2 | 5 | 13:21 | -8 | 8 |
| 16 |
|
FC Sellier & Bellot Vlašim | 9 | 2 | 0 | 7 | 13:19 | -6 | 6 |
| Promotion | |
| Play-off | |
| Relégation |
