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| 10.03.2023 | 17:00 | FK Viagem Příbram | - | SK Líšeň | 2:0 (1:0) | |
| 11.03.2023 | 10:15 | MFK Vyškov | - | SFC Opava | 1:1 (1:0) | |
| 14:30 | FC Sellier & Bellot Vlašim | - | AC Sparta Praha B | 3:0 (1:0) | ||
| 12.03.2023 | 10:15 | Slavia Praha B | - | FK Třinec | 3:1 (1:1) | |
| 14:30 | Vysočina Jihlava | - | MFK Karviná | 2:2 (1:1) | ||
| 13.03.2023 | 16:00 | Sigma Olomouc B | - | 1. SK Prostějov | 2:0 (1:0) | |
| 25.03.2023 | 14:00 | MFK Chrudim | - | FK MAS Táborsko | 1:1 (1:1) | |
| 04.04.2023 | 16:30 | FK Varnsdorf | - | FK Dukla Praha | 3:4 (0:3) |
| » Classement | équipe recevante | à l'extérieur | Premier tour | Second tour |
| # | Équipe | M. | G. | N. | P. | Buts | Diff. | Pts. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Viagem Příbram | 18 | 9 | 7 | 2 | 31:13 | 18 | 34 |
| 2 |
|
MFK Vyškov | 18 | 8 | 7 | 3 | 30:18 | 12 | 31 |
| 3 |
|
SK Líšeň | 18 | 9 | 4 | 5 | 25:13 | 12 | 31 |
| 4 |
|
MFK Karviná | 18 | 9 | 2 | 7 | 31:26 | 5 | 29 |
| 5 |
|
FK Varnsdorf | 18 | 8 | 4 | 6 | 29:23 | 6 | 28 |
| 6 |
|
MFK Chrudim | 18 | 6 | 7 | 5 | 21:18 | 3 | 25 |
| 7 |
|
FK Dukla Praha | 18 | 7 | 3 | 8 | 27:28 | -1 | 24 |
| 8 |
|
Sigma Olomouc B | 18 | 6 | 6 | 6 | 22:29 | -7 | 24 |
| 9 |
|
Vysočina Jihlava | 18 | 6 | 5 | 7 | 20:26 | -6 | 23 |
| 10 |
|
1. SK Prostějov | 18 | 5 | 7 | 6 | 28:34 | -6 | 22 |
| 11 |
|
FK MAS Táborsko | 18 | 5 | 7 | 6 | 16:23 | -7 | 22 |
| 12 |
|
FC Sellier & Bellot Vlašim | 18 | 6 | 3 | 9 | 30:29 | 1 | 21 |
| 13 |
|
SFC Opava | 18 | 5 | 6 | 7 | 17:17 | 0 | 21 |
| 14 |
|
AC Sparta Praha B | 18 | 6 | 2 | 10 | 17:27 | -10 | 20 |
| 15 |
|
Slavia Praha B | 18 | 5 | 4 | 9 | 25:35 | -10 | 19 |
| 16 |
|
FK Třinec | 18 | 4 | 6 | 8 | 19:29 | -10 | 18 |
| Promotion | |
| Play-off | |
| Relégation |
