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| 26.08.2016 | 20:30 | KFC Oosterzonen | - | Union Saint-Gilloise | 3:2 | |
| 20:30 | KFCO Beerschot Wilrijk | - | Cercle Brugge | 1:2 | ||
| 27.08.2016 | 17:00 | KFC Helson Helchteren | - | Eendracht Aalst | 1:2 | |
| 19:00 | SK Deinze | - | Lommel United | 1:3 | ||
| 19:00 | Torhout 1992 KM | - | AFC Tubize | 0:4 | ||
| 19:30 | Sprimont Comblain | - | FC Turnhout | 7:6 tab | ||
| 19:30 | Bocholter VV | - | KSV Oudenaarde | 4:2 ap | ||
| 19:30 | Patro Eisden MM | - | KFC Dessel Sport | 0:6 | ||
| 20:00 | Stade Waremmien | - | Lierse SK (old) | 2:8 | ||
| 20:00 | Royal Antwerp FC | - | KFC Zwarte Leeuw | 4:1 | ||
| 28.08.2016 | 16:00 | St. Eloois-Winkel Sport | - | ASV Geel | 0:1 | |
| 16:00 | SK Londerzeel | - | KSV Roeselare | 1:2 | ||
| 16:00 | RC Charleroi-Couillet-Fleurus | - | KRC Harelbeke | 0:1 | ||
| 16:00 | RFC Meux | - | R. Cappellen FC | 4:5 | ||
| 16:00 | RUS Rebecquoise | - | Oud-Heverlee Leuven | 3:5 tab | ||
| 16:00 | Sportkring Sint-Niklaas | - | Sporting Hasselt | 0:1 |
